Five xMAP® Technology Tips from Luminex Tech Support

Whether you’re a new or experienced xMAP® Technology user, our Technical Support Team can help you! They’ve compiled five tips based on questions that users frequently ask.

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Tip #1: Frustrated by verification failure?

Some ® 200™” href=”/luminex-100200/”>Luminex® 200™ and ® Kit Finder” href=”https://www.luminexcorp.com/kitfinder”>xMAP® Kit Finder and the xMAP Cookbook, Linscott’s Directory is a great resource, with over 2,100,000 unique antibody listings and 1,000,000 other immunological and biological reagents.

Tip #4: Does your quantitative protein assay lack sensitivity?

Try increasing the primary incubation. Primary incubation times to test are one hour, two hours, and overnight. One and two hour incubations can be performed at room temp while shaking. Overnight incubations should be performed while refrigerated and shaking.

Tip #5: Want stellar protein coupling?

Before coupling proteins (including antibodies) to beads, verify the protein concentration via NanoDrop™, bicinchoninic acid (BCA) assay, or Bradford assay. While we don’t suggest a specific concentration, it’s always best to verify the protein concentration that’s on the label or in the certificate of analysis, as they aren’t always correct.

Take a look at our Customer Center, where you’ll find contact information, product documents, target values and Certificates of Quality, training resources, and more.

Have a question and don’t know where to start? View this flyer to see how we can help.

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